SLU-PP-332 – Technical Brief (Research-Use-Only)
1. Identity
SLU-PP-332 (4-hydroxy-N-[(Z)-naphthalen-2-ylmethylidene]-benzamide) is a synthetic, yellow crystalline small-molecule discovered at Saint Louis University. It is a potent, non-selective agonist of the estrogen-related receptors (ERRs), displaying its greatest activity at ERR-α with an EC₅₀ ≈ 98 nM A B. Because it is not peptide-based, it shows good metabolic stability and can penetrate cells without a delivery vector.
Property Detail
Molecular formula C₁₈H₁₄N₂O₂
Molar mass 290.3 g mol⁻¹
Appearance Yellow powder
Water solubility < 0.1 mg mL⁻¹; freely soluble in DMSO/DMF A
CAS 303760-60-3
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2. Mechanistic Highlights
1. ERR-α/β/γ activation – Binding to the ERR ligand-binding domain locks the receptor in its active conformation and strengthens interaction with the co-activator PGC-1α A.
2. Mitochondrial biogenesis – Up-regulation of TFAM and other nuclear-encoded OXPHOS genes increases mitochondrial number and respiratory capacity.
3. Enhanced substrate utilisation – Promotes fatty-acid uptake (↑ CPT-1, MCAD) and β-oxidation, while improving glucose disposal, mimicking many exercise-induced transcriptional changes B.
4. Thermogenesis – Activation of ERR pathways elevates uncoupling protein expression, raising basal energy expenditure in rodent models.
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3. Safety Profile (pre-clinical)
There are no published human data. Rodent studies using 5–20 mg kg⁻¹ day⁻¹ (i.p. or oral gavage) for ≤ 14 days have not reported overt toxicity, but full GLP toxicology is lacking. The compound may cause skin/eye/respiratory irritation; handle in a fume hood and avoid aerosol generation A.
These findings do not establish safety for unregulated human exposure. SLU-PP-332 is supplied strictly for laboratory research purposes.
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4. Legal Position in the United Kingdom
• SLU-PP-332 is not scheduled under the Misuse of Drugs Act and is not a controlled substance.
• When sold and labelled “FOR RESEARCH USE ONLY – NOT FOR HUMAN OR VETERINARY USE,” it sits outside the Human Medicines Regulations 2012.
• Any marketing that implies therapeutic, performance-enhancement, or weight-loss use reclassifies it as a medicinal product, invoking MHRA licensing.
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5. Practical Laboratory Handling
Reconstitution: use sterile 0.9 % Benzyl alcohol Bacteriostatic water, 1–3 mL⁻¹; swirl gently—avoid vigorous shaking.
• Storage: aliquot, −80 °C long term; ≤ 30 days at −20 °C once re-suspended.
• Typical exploratory dosing (rodent): 250-500mcg subcutaneously, Once or twice Daily.
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Bottom Line
SLU-PP-332 is an ERR agonist valued for investigating metabolic rewiring and exercise-mimetic pathways. In the UK it may be purchased, possessed, and employed solely as a laboratory research reagent; any promotion or use beyond this remit contravenes medicines legislation.
